Biohydrogen, a promising component of biofuels, is produced through biological processes using microorganisms such as algae, bacteria, and archaea. These organisms utilize sunlight, organic matter, or other renewable resources to produce hydrogen gas as a byproduct of their metabolic activities. Unlike conventional fossil fuels, biohydrogen is renewable, emits no greenhouse gases, and can be produced from various organic materials including agricultural waste, sewage sludge, and industrial byproducts. Its production not only reduces dependence on finite fossil fuels but also addresses environmental concerns associated with their combustion. Despite its potential, challenges such as low production efficiency and high costs hinder widespread adoption. Research into optimizing production processes and exploring novel biohydrogen sources holds promise for its integration into the sustainable energy landscape, offering a cleaner alternative to conventional fuels.