One of the most promising non-food feedstocks, lignocellulosic biomass for biofuel production, offers an abundant and renewable energy source. Composed of cellulose, hemicellulose, and lignin, its complex structure presents challenges in efficient breakdown and conversion. Recent advances in pretreatment methods, including steam explosion, acid hydrolysis, and enzymatic digestion, have improved sugar release for bioethanol fermentation. Additionally, lignin valorization is gaining attention for producing bio-based chemicals and fuels, increasing overall process viability. Innovations in microbial engineering and consolidated bioprocessing are streamlining conversion pathways, enhancing yields, and lowering costs. As sustainability remains a priority, further refining these technologies is essential for making lignocellulosic biofuels a scalable alternative to fossil-based energy sources.